Evaluation of bacterial contamination of wastewater treatment plants at Agadir (Morocco): isolation, molecular characterization and antibiotic resistance of Vibrio species

EDDABRA, Rkia (2011) Evaluation of bacterial contamination of wastewater treatment plants at Agadir (Morocco): isolation, molecular characterization and antibiotic resistance of Vibrio species. Thèses de doctorat, Université de Strasbourg.

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Abstract

In countries such as Morocco, characterized by scarce rainfall, lack of freshwater resources and high groundwater salinity where agriculture reuse of municipal wastewater is becoming a compulsory choice for water resources management. This study evaluated the efficiency of the three wastewater treatment plants (WTPs) for the removal of physico-chemical and microbiological contaminants. Resistance to antibiotics, pulsed field gel electrophoresis and MALDI-TOF mass spectrometry were used to characterize Vibrio isolates. Mean values were used to determine treatment performances of the 3 WTPs, electric conductivity didn’t showed any difference between raw and treated wastewater and the mean values varying from 2900 μS/cm to 3300, the pH varying from 6,66 to 8,6, and the temperature varying between 16°C and 26.4°C. Removal efficiencies of fecal coliforms, enterococci and spores of sulphite reducing anaerobic bacteria were betwenn 3 and 4 log unit for the 3WTPs. 4 species of Vibrio were identified, among the 58 Vibrio sp. isolated, 53,44% were identified as V. cholerae, 29,31% as V. alginolyticus, 17,78% as V. fluvialis and 1,74% as V. metschnikovii. Of the total 58 Vibrio isolates, 53,44% were susceptible to all antibiotics. Of the resistant (46,56%) Vibrio strains, 39,83% were resistant against one to three antibiotics. PFGE with NotI digestion produced patterns with higher level of heterogeneity, and about 31% of Vibrio isolates were untypeable. MALDI-TOF-MS-based fingerprinting of Vibrio isolates has potential as a rapid for identification and finest differences between strains can readily be evaluated by the dendrogram based on percentage identity of MALDI-TOF mass spectra of Vibrio isolates, but requires further development for database of BioTyper.

Item Type:	Thesis
Discipline de la thèse / mémoire / rapport :	Microbiologie
Uncontrolled Keywords:	eaux usées ; station d’épuration ; Vibrio ; bactéries fécales ; résistance aux antibiotiques ; spectrométrie de masse MALDI-TOF ; électrophorèse en champ pulsé ; physico-chimie
Subjects:	CL Classification > DDC Dewey Decimal Classification > 600 Technologie (sciences appliquées) > 620 Sciences de l'ingénieur > 628 Technique sanitaire et municipale. Génie urbain > 628.3 Traitement/élimination des eaux usées Classification Thèses Unistra > Sciences, technologies > Sciences appliquées > 620 Sciences de l'ingénieur > 628 Technique sanitaire et municipale. Génie urbain > 628.3 Traitement/élimination des eaux usées UNERA Classification UNERA > ACT Domaine d'activité UNERA > ACT-14 Eau, environnement, aménagement, urbanisme UNERA Classification UNERA > DISC Discipline UNERA > DISC-16 Sciences de la vie et de la santé, psychologie
ID Code:	2333
Deposited On:	22 February 2012

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