New data concerning endogenous morphine : description of its localization in the central nervous system and development of biotechnological and therapeutic tools
LAUX, Alexis
(2011)
New data concerning endogenous morphine : description of its localization in the central nervous system and development of biotechnological and therapeutic tools.
Thèses de doctorat, Full text available as:
AbstractSince 80s, endogenous morphine (eM), structurally identical to the morphine from poppy, has been found in mammalian central nervous system (CNS). Only few informations are available about eM (cellular localization, models and quantification protocols) and thus, studies of its roles and implications in the brain physiology is difficult. During my thesis, my goal was to developed new tools in order to study and understand the roles of eM in the CNS. First, I have described the localization of eM in the CNS of adult mouse. Using a well characterized antibody, I have demonstrated that eM and its derived compounds are mainly found in astrocytes and GABAergic interneurons throughout the mouse CNS. Secondly, I described two endogenous / exogenous alkaloids binding proteins. (i) The PhosphatidylEthanolamine Binding Protein that is able to bind M6G and M3G in a similar manner as its reference ligand PhosphatidylEthanolamine. (ii) The creatine kinase (CK) that is able to bind eM and its derived compounds with high affinity. Such CK-eM complex is dissociated by a lithium treatment. Finally, in the last part of my results, I have described that the presence of lithium in the collection tube allows a better measuring of eM and exogenous morphine. To conclude, during my thesis I set up new tools and new research lines (eM CNS mapping, binding proteins and better quantification yield) that will allow to study and understand the roles of eM in the CNS of mammals. Repository Staff Only: edit this item |
